RNA
miRts luc

Part:BBa_J176025

Designed by: Karmella Haynes   Group: Haynes Lab   (2011-09-29)

miRts luc

miRNA target for luciferase (artificial RNAi system)



Usage and Biology

  • Put this sequence in the 3' UTR of a gene to place it under the control of miR-luc


This sequence contains 5 repeats that have partial complementarity with an artificial microRNA (from firefly luciferase gene). This target site is intended to mimic the natural microRNA silencing pathway (as opposed to siRNA silencing). The luciferase RNAi system is orthogonal to mammalian cell RNAi pathways, so it should not have any cross-talk with natural miRNAs/ targets.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 21
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/amiRts luc